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For rescued expression of GLUT1 or DHHC9 in GLUT1 and DHHC9-knockout cells, respectively, Flag-tagged sgRNA-resistant (r) wild-type GLUT1 or rDHHC9 or their mutants (rGLUT1 C207S and rDHHC9 C169S) were cloned into pCDH lentiviral vector selectable marker of hygromycin
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