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Methylcobalamin: Essential for brain and nerve function, red blood cell production, and DNA synthesis

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Some of the right lungs were harvested and stored at -80°C for subsequent analysis by real time quantitative polymerase chain reaction (qPCR) and western blotting, while other right lungs were perfused with cryomatrix (Thermo, New York, NY, United States) for immunofluorescence staining