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Genotyping was performed by PCR with the following primer sets, resolved by electrophoresis on 0.5X TBE agarose gels (1.0–2.0%): (1) For Cacna1a (Ca V 2.1) KO, a single multiplexed PCR was performed for each animal with four primers targeting exon 4 of Cacna1a , deleted in the mutant line: 5′-CGTTCCTTGCGCACGTGTGCT C-3′ 5′-GGGATCATCGCCTTCATGATTGACTTCAGGACGA CT-3′
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