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Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml −1 )/streptomycin (100 µg ml −1 )/fungizone (0.25 µg ml −1 )

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Vitamin B12 Solution 1000mcg/ml (Quantity):9 x 10ml

However, like MVD measurements, a negative effect on vascular volume indicated by non-invasive imaging cannot be interpreted as absence of antiangiogenic effect, either (190)