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Cellular debris was removed from the resultant pool by centrifugation at 3270 rcf for 25 min at 4 °C, and supernatant was pumped through 1000 cm 2 , 0.8/0.2 µm filters (12992, Pall) to create RP-HCCF

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First and foremost, they can help alleviate the symptoms associated with B12 deficiency, such as loss of appetite, weight loss, and gastrointestinal issues