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Development of a tool for correlational analyses Since a secondary aim of this study was to examine potential relationships between the cecal microbiome and adipose tissue metabolome, a Python (v3.8) tool, which used modules from SciPy 39 , was created to expedite the correlational analyses between the cecal microbiome and the previously-published epididymal white adipose tissue (EWAT) metabolome 13

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By inhibiting NNMT, 5-Amino-1MQ increases cellular SAM and NAD+ levels, enhances NAD+ salvage pathway flux, activates SIRT-1, boosts oxidative phosphorylation (OXPHOS), and influences cellular energy homeostasis and epigenetic modifications