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This Genistein-encapsulated DDS demonstrated the most favorable outcomes in relieving Alzheimer’s disease symptoms in vitro and in vivo via the synergistic action of the combination of MA membranes RVG29 and TPP [155]
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Inhibition assay of cysteine cathepsin proteases The cathepsin B inhibitor screening assay 57 uses the ability of cathepsin B to cleave the synthetic AMC (7-amino-4-methylcoumarin)-based peptide substrate to release AMC, which can be quantified using a fluorometer or fluorescence microplate reader
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