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MRAB-3 Growth on Pre-confluence HFF-1 Cells To study the effect of MRAB-3 on pre-confluence of HFF-1 cells, HFF-1 cells were cultured and harvested as above

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We show that gspD is an essential gene in Myxococcus xanthus , and its downregulation by conditional knockdown renders this bacterium defective in both slime secretion and gliding motility