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Sample analysis was performed on an Agilent 1290 UPLC system equipped with a Waters BEH amide (2.1 mm × 100 mm, 1.7 μm) column using a gradient of 15 mM NH₄Ac/0.3% NH₄OH in H₂O (A) and 90% ACN (B) at 0.3 mL/min

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The Cagri-Sema blend is a dual-component research formulation combining two complementary metabolic peptides: cagrilintide, a long-acting amylin receptor agonist, and semaglutide, a GLP-1 receptor agonist