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doi: 10.1016/j.cca.2009.06.028
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In situ hybridization In situ hybridization (ISH) was performed on fresh frozen coronal brain sections (25 µm) according to protocols that are available at Briefly, digoxigenin (DIG)-labeled RNA probes were transcribed in vitro from cloned cDNAs for mouse Pdgfra, Enpp6, Plp, Mbp and Mog as previously described (Huang et al., 2018
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